What is the course about?

Nuclear magnetic resonance spectroscopy or NMR spectroscopy is the most informative analytical method for studying the structure of organic molecules, and is also used for their direct quantitative determination even in complex mixtures. Therefore, it is widely used for identification and quantification of biologically active substances, auxiliary substances and impurities in pharmaceutical analysis. NMR spectroscopy combines a unique set of advantages: simple sample preparation, rapidity, universality, precision, the possibility of relative and absolute quantitative determination of components without authentic reference compounds. In addition, NMR spectroscopy is a non-destructive method, and after recording the NMR spectrum, the analyte can be analyzed by other methods.

Who is this course for?

The course is primarily addressed to students studying in the specialties “Pharmacy” and “Chemistry”, as well as specialists with higher education, working in pharmaceutical and chemical field.

What do I need to know?

It is necessary to know the basics of organic, analytical chemistry and also to be able to work with information sources.

What will you have learnt?

By the end of the course you will know: theoretical basis of ¹H NMR spectroscopy; the relationship of the ¹H NMR spectra parameters with the structure of molecules; identification of molecules using ¹H NMR spectra; the effect of physico-chemical properties on quantitative analysis; possibilities of ¹H NMR spectroscopy in the quantitative analysis of simple and complex organic systems; rules for the creation and validation of quantitative ¹H NMR spectroscopy techniques.

Course Structure

Lecture 1. Instrumental methods of pharmaceutical analysis.

Lecture 2. Basiсs of ¹H NMR spectroscopy.

Lecture 3. Basics of quantitative ¹H NMR spectroscopy. Physico-chemical properties affecting quantitative NMR analysis.

Lecture 4. Basics of quantitative ¹H NMR spectroscopy. Referencing techniques.

Lecture 5. Basics of quantitative ¹H NMR spectroscopy. Experimental parameters affecting quantitative NMR analysis.

Lecture 6. Validation of quantitative NMR spectroscopy techniques.

Lecture 7. Quantitative NMR spectroscopy in pharmaceutical chemistry.

Lecture 8. Quantitative NMR spectroscopy in pharmacognosy.

The team and organisation standing behind the course

Vasil’ev Vasilii Gennad’evich, PhD, Deputy Director of the Center for Precision Instrumental Methods of Analysis “PRIMA” of the Shared Research and Educational Center of RUDN University.

Kalabin Gennady Aleksandrovich, Dr.Sci., Professor of the Department of System Ecology, Faculty of Ecology of RUDN University, Director of the Center for Precision Instrumental Methods of Analysis “PRIMA” of the Shared Research and Educational Center of RUDN University.

Additional literature for self-study

Mitchell T.N., Costisella B. NMR – From Spectra to Structures. An Experimental Approach. 2nd Edition. Springer. 2007. P. 220.
Holzgrabe U., Wawer I., Diehl B. NMR Spectroscopy in Pharmaceutical Analysis. 1st Edition. Elsevier. 2008. P. 528.
United States Pharmacopeia (USP) 38 – NF 33. 2015.
European Pharmacopoeia (Ph. Eur.) 9th Edition. EDQM Council of Europe. 2016.

Course instructors

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Vasilii Vasil'ev

Born in 1988. He graduated from the Faculty of Sciences of RUDN University in 2014, specialty "Chemistry".
In 2014 - 2017, he was a graduate student at the RUDN University.
Since 2014, he has been working as Deputy Director of the Center for Precision Instrumental Methods of Analysis “PRIMA” of the Shared Research and Educational Center of RUDN University.
In 2018, he defended his thesis on the specialty “Pharmaceutical chemistry, pharmacognosy” on the topic “Quality control of drugs and plant-based objects by 1H NMR spectroscopy without using of reference compounds”.

Quantitative NMR spectroscopy in pharmaceutical chemistry and pharmacognosy